D-amino acid oxidase

D-amino acid oxidase

D-amino-acid oxidase
3D structure of DAAO from yeast (monomer)
Identifiers
EC number 1.4.3.3
CAS number 9000-88-8
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO
D-amino-acid oxidase
Identifiers
Symbol DAO (DAAO)
Entrez 1610
HUGO 2671
OMIM 124050
RefSeq NM_001917
UniProt P14920
Other data
EC number 1.4.3.3
Locus Chr. 12 q24

D-amino acid oxidase (DAAO; also DAO, OXDA, DAMOX) is a peroxisomal enzyme containing FAD as cofactor that is expressed in a wide range of species from yeasts to human.[1] It is not present in plants or in bacteria which instead use D-amino acid dehydrogenase. Its function is to oxidize D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide.

This enzyme belongs to the FAD dependent oxidoreductase family, and acts on the CH-NH2 group of D-amino acid donors with oxygen as acceptor. The enzyme is most active toward neutral D-amino acids, and not active toward acidic D-amino acids.

Recently, mammalian D-amino acid oxidase has been connected to the brain D-serine metabolism and to the regulation of the glutamatergic neurotransmission. In a postmortem study, the activity of DAAO was found to be two-fold higher in schizophrenia.[2]

DAAO is a candidate susceptibility gene[3] and together with G72 may play a role in the glutamatergic mechanisms of schizophrenia.[4] Risperidone and sodium benzoate are inhibitors of DAAO.

DAAO is used as a biocatalyst in several biotechnological applications, such as the oxidation of cephalosporin C, the deracemition of racemic D-amino acid solutions and as the biological component in several biosensors for the determination of the content in D-amino acids of biological fluids.

This protein may use the morpheein model of allosteric regulation. [5]


See also

External links

  • D-Amino-Acid Oxidase at the US National Library of Medicine Medical Subject Headings (MeSH)
  • http://www.calzyme.com/commerce/catalog/spcategory.jsp?category_id=1043

References

  1. ^
  2. ^
  3. ^ Gene Overview of All Published Schizophrenia-Association Studies for DAAO - SZGene database.
  4. ^
  5. ^