10-week-old human fetus surrounded by amniotic fluid within the amniotic sac
Amniotic fluid or liquor amnii is the protective liquid contained by the amniotic sac of a pregnant female.
- Contents 1.1
- Volume 1.2
- Rupture of membranes 1.3
- Function 2
Clinical significance 3
- Analysis 3.1
- Complications related to amniotic fluid 3.2
- Stem cell research 3.3
- See also 4
- References 5
Amniotic fluid is present from the formation of the gestational sac. Amniotic fluid is present in the amniotic sac. It is generated from maternal plasma, and passes through the fetal membranes by osmotic and hydrostatic forces. When fetal kidneys begin to function in about week 16, fetal urine also contributes to the fluid.
The fluid is absorbed through the fetal tissue and skin. After the 20th-25th week of pregnancy when the keratinization of an embryo's skin occurs, the fluid is primarily absorbed by the fetal gut.
At first, amniotic fluid is mainly water with electrolytes, but by about the 12-14th week the liquid also contains proteins, carbohydrates, lipids and phospholipids, and urea, all of which aid in the growth of the fetus.
The volume of amniotic fluid increases with the growth of fetus. From the 10th to the 20th week it increases from 25ml to 400ml approximately. Approximately in the 10th week the breathing and swallowing of the fetus slightly decrease the amount of fluid, but neither urination nor swallowing contributes significantly to fluid quantity changes, until the 25th week, when keratinization of skin is complete. Then the relationship between fluid and fetal growth stops. It reaches a plateau of 800ml by the 28 week gestational age. The amount of fluid declines to roughly 400 ml at 42 weeks. There is about 1L of amniotic fluid at birth.
Rupture of membranes
The forewaters are released when the amnion ruptures. This is commonly known as the time when a woman's "water breaks". When this occurs during labour at term, it is known as "spontaneous rupture of membranes". If the rupture precedes labour at term, however, it is referred to as "premature rupture of membranes". The majority of the hindwaters remain inside the womb until the baby is born. Artificial rupture of membrane (ARM), a manual rupture of the amniotic sac, can also be performed to release the fluid if the amnion has not spontaneously ruptured.
Swallowed amniotic fluid creates urine and contributes to the formation of meconium. Amniotic fluid protects the developing baby by cushioning against blows to the mother's abdomen, allowing for easier fetal movement and promoting muscular/skeletal development. Amniotic fluid swallowed by the fetus helps in the formation of the gastrointestinal tract. Contrary to popular belief, amniotic fluid has not been conclusively shown to be inhaled and exhaled by the fetus. In fact, studies from the 1970's show that in a healthy fetus, there is no inward flow of amniotic fluid into the airway. Instead, lung development occurs as a result of the production of fetal lung fluid which expands the lungs.
Analysis of amniotic fluid, drawn out of the mother's abdomen in an amniocentesis procedure, can reveal many aspects of the baby's genetic health. This is because the fluid also contains fetal cells, which can be examined for genetic defects.
Amniotic fluid normally has a pH of 7.0 to 7.5. Because pH in the upper vagina is normally acidic (pH 3.8-4.5), a vaginal pH test showing a pH of more than 4.5 strengthens a suspicion of rupture of membranes in case of clear vaginal discharge in pregnancy. Other tests for detecting amniotic fluid mainly include nitrazine paper test and fern test.
Too little amniotic fluid (oligohydramnios) can be a cause or an indicator of problems for the mother and baby. The majority of pregnancies proceed normally and the baby is born healthy, but this isn't always the case. Babies with too little amniotic fluid can develop contractures of the limbs, clubbing of the feet and hands, and also develop a life-threatening condition called hypoplastic lungs. If a baby is born with hypoplastic lungs, which are small underdeveloped lungs, this condition is potentially fatal and the baby can die shortly after birth due to inadequate oxygenation. Potter sequence refers to a constellation of findings related to insufficient amniotic fluid and includes shortened and malformed limbs with clubbed feet and the underdeveloped lungs that can lead to perinatal death.
On every prenatal visit, the obstetrician/gynaecologist or midwife should measure the patient's fundal height with a tape measure. It is important that the fundal height be measured and properly recorded to track proper fetal growth and the increasing development of amniotic fluid. The obstetrician/gynaecologist or midwife should also routinely ultrasound the patient—this procedure will also give an indication of proper fetal growth and amniotic fluid development. Oligohydramnios can be caused by infection, kidney dysfunction or malformation (since much of the late amniotic fluid volume is urine), procedures such as chorionic villus sampling (CVS), and preterm premature rupture of membranes (PPROM). Oligohydramnios can sometimes be treated with bed rest, oral and intravenous hydration, antibiotics, steroids, and amnioinfusion. It is also important to keep the baby warm and moist.
The opposite of oligohydramnios is polyhydramnios, an excess volume of amniotic fluid in the amniotic sac.
Stem cell research
Recent studies show that amniotic fluid contains a considerable quantity of stem cells. These amniotic stem cells are pluripotent and able to differentiate into various tissues, which may be useful for future human application. Some researchers have found that amniotic fluid is also a plentiful source of non-embryonic stem cells. These cells have demonstrated the ability to differentiate into a number of different cell-types, including brain, liver and bone.
It is possible to conserve the stem cells extracted from amniotic fluid in private stem cells banks. Some private companies offer this service for a fee.
- Larsen, William J. (2001). Human embryology (3. ed.). Philadelphia, Pa.: Churchill Livingstone. p. 490.
- Underwood, Mark A; Gilbert, William M; Sherman, Michael P (24 March 2005). "Amniotic Fluid: Not Just Fetal Urine Anymore". Journal of Perinatology 25 (5): 341–348.
- Forewaters and hindwaters in Q&A section at babyworld.co.uk
- Lily A.W. Disorder of Amniotic Fluid: ASSALI, N.S. Pathophysiology of Gestation Volume II. Academic Press, New York & London. 1972
- Vaginal pH Test from Point of Care Testing, July 2009, at: University of California, San Francisco – Department of Laboratory Medicine. Prepared by: Patricia Nassos, PhD, MT and Clayton Hooper, RN.
- Bennett, S.; Cullen, J.; Sherer, D.; Woods Jr, J. (2008). "The Ferning and Nitrazine Tests of Amniotic Fluid Between 12 and 41 Weeks Gestation". American Journal of Perinatology 10 (2): 101–104.
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- "Isolation of amniotic stem cell lines with potential for therapy", Nature Biotechnology 25, 100 - 106 (2007), Published online: 7 January 2007 | doi:10.1038/nbt1274, retrieved 27 July 2009 
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- "Amniotic Fluid Yields New Type of Stem Cell", PBS - The Online News Hour, Jan 8 2007, retrieved 27 July 2009 
- "Versatile Stem Cell Identified in Amniotic Fluid", Pamela J. Hines, International Society of Stem Cell Research, March 21, 2008, retrieve 27 July 2009 
- "Amniotic Stem Cells - "Mesenchimal Stem Cells in Human Application", Biocell Center Group, 2009, retrieved 27 July 2009 
- "Isolation of amniotic stem cell lines with potential for therapy : Abstract : Nature Biotechnology". Retrieved 2007-10-13.