TNP is excited at a wavelength of 408 and 470 nm, and fluoresces in the 530-560 nm range. When TNP-ATP is in solution but not bound to a protein, this emission is very weak. However, once TNP-ATP binds to a protein, the TNP fluorescence is markedly enhanced. Thus, with enhanced fluorescence, it can be seen whether or not a protein binds to ATP.
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